glycine silver impregnation kit, 200 mL×4+150 mL+250 mL+250 mL (for Nervous tissue staining)

Product Information

Description

This kit is suitable for nerve fiber staining，which is based on the principle that axons of nerve fibers are silver-philic. After the nerve tissue section is treated with acid formaldehyde solution, it reacts with silver glycine solution to deposit silver ions on the silver-philic axons, and then the reducing agent is used to reduce silver ions to black elemental silver so that axons can be displayed. This method is applicable to paraffin section or frozen section staining of fixed nerve tissue, which is often used to study and observe the pathological changes of nerve cell fibrils in the brain of senile dementia and non-dementia elderly patients, or to observe whether there is degeneration, damage degree and scope of peripheral nerves (such as leprosy). After staining, axons, intracellular fibrils, neurofibrillary tangles, senile plaques and dendrites were black with a golden background. This method can also be used to differentiate neurofibroma from schwannoma, which is positive for neurofibroma and negative for schwannoma. Kit composition: staining solution A is pyrogallic acid reducing solution, staining solution B is silver glycine solution, staining solution C is acid formaldehyde, and staining solution D is sodium thiosulfate solution.

Storage and Handling Conditions

Transport at room temperature; Keep out of light, valid for 12 months.

Component

Assay Protocol

1. Paraffin sections are dewaxed and rehydrated, and washed 3-5 times with distilled water.

2. Silver glycine staining solution C staining: Sections were treated with silver glycine staining solution C for 5min and washed with distilled water for 3 times.

3. Silver glycine staining solution B treatment: The sections were put into silver glycine staining solution B (preheated at 37 in advance) and treated for 3-5min.

4. Silver glycine staining solution A treatment: Remove slices, quickly get rid of tissue remnants of glycine silver staining solution B, into the glycine silver staining solution A (15 min in 45 preheat in advance), observing reduction effect, after A few seconds to take out the sliced and quickly shaken to slice into fresh glycine silver staining solution A (15 min in 45 preheat in advance), after A few seconds out quickly, Wash with distilled water. If the background is too deep, it needs to be treated with silver glycine staining solution D and washed with distilled water for 3 times.

5. Dehydration and mounting: The slices were dehydrated with absolute ethanol for 3 times, 5 min each time; Xylene was transparent for 5 min, then fresh xylene was transparent for 5 min, and the slices were mounted with neutral gum.

Note:

1. The steps that need washing in the dyeing process must be cleaned with distilled water, not tap water, otherwise impurities are easy to appear.

2. Avoid light during operation. It is recommended to make one slice each time and replace the staining solution with a new one each time.

3. Wear a lab coat and disposable gloves during operation.

For Research Use Only!

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