Products
Pathological Reagents | Consumables
Morphology Staining Solution
Special Stains
Nerve Tissue Stain
SKU : G1036-100ML
หมวดหมู่ : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat.No. | Spec. |
Nissl Stain Solution | G1036-100ML | 100 mL |
Description
Nissl staining solution, commonly used in neurobiology, is used for Nissl body staining in nerve cells. Nissile bodies, which are mainly composed of ribosomes and rough endoplasmic reticulum, are basophilic. Nissl body can be used as a sensitive marker of nerve cell injury in histomorphology. Therefore, Nissl staining can be used to show the basic neural structure of brain or spinal cord and reflect the pathological morphology of nerve cells. The number of Ronei bodies and normal morphology indicate that the function of nerve cells is normal. The decrease in the number of Ronei bodies and abnormal morphology indicate that nerve cells may be damaged.
The main component of this product is toluidine blue, the concentration is 0.5%, can be used for Nissl staining, the operation is simple and fast. After staining, the dark blue granules of Nissilium were visible, and the nuclei were pale blue. The background was basically colorless.
Storage and Handling Conditions
Store and transport at room temperature, valid for 18 months.
Component
Component | G1036-100ML |
Nissl Stain Solution | 100 mL |
Product Manual | |
Assay Protocol
1. Sample processing
For paraffin sections, the sections were dewaxed by xylene for 5-10 min, and then dewaxed again by adding fresh xylene for 5-10 min. Then slice into absolute ethanol for 5 min, fresh absolute ethanol for 5 min, then treated with 75% ethanol for 5 min, and washed with water for 2 min.
For frozen sections: Remove the section from -20 ° C and return to room temperature. If fixation is required, fix with 4% paraformaldehyde (G1101 is recommended) at room temperature for at least 10 min and wash with water for 2 min.
For cultured cells, cells were fixed with 4% paraformaldehyde (G1101 recommended) for at least 10 min and washed with water for 2 min.
2. Nissl staining: The above processed samples should be stained with Nissl staining solution for 2-5 min (the time can be adjusted according to your dyeing requirements). Wash until the slide has no color in running water.
3. (optional) Differentiate the sections with 0.1% glacial acetic acid. Appropriate differentiation or nondifferentiation of the tissue should be implemented according to the color of the tissue. Brain tissue differentiated to Nissile bodies is dark blue with a light blue or colorless background.
4. Dry the excess water on the slices and place the slices in the oven at 65for 4h or more to dry.
5. Sufficiently baked and immersed in xylene for 10 min in clear or leave the slides to cool in xylene and seal them with neutral gum.
Note: Prepare xylene, gradient ethanol, 0.1% glacial acetic acid, neutral gum, etc.
Note:
1. Wear a lab coat and disposable gloves during operation.
2. Staining Solution is reusable. 100 mL of Staining Solution can be used to stain (dip or drop) approximately 350 sections. Replace the staining solution with a new one when the tissue or cell coloring is significantly lighter or abnormal.
For Research Use Only!
