Dithiothreitol, 1 g (for Sugar Alcohols)

Product Information

Introduction

Dithiothreitol (DTT) is a small molecule organic reducing agent with chemical formula of C4H10O2S2. It is a linear molecule in the reduced state and becomes a six membered ring structure containing disulfide bonds after being oxidized. The name dithiothreitol is derived from threitol (a four-carbon monosaccharide). The isomer of DTT is dithiocarbitol (DTE), which is the C3 epimer of DTT.

Basic Attributes

Storage and Handling Conditions

Store at 2-8，ship with wet ice, valid for 48 months.

Instruction

One of the uses of DTT is as a reducing agent and deprotective agent for thiolated DNA. Thiolated DNA terminal sulfur atoms tend to form dimers in solution, especially in the presence of oxygen. This dimerization greatly reduces the efficiency of some coupling reaction experiments (such as DNA fixation in biosensors); The dimerization of DNA can be reduced by adding DTT to the DNA solution and removing it after a period of reaction. DTT is also often used to reduce disulfide bonds in proteins, and can be used to prevent intramolecular or intermolecular disulfide bonds formed between cysteines in proteins. However, DTT is often unable to reduce the disulfide bonds embedded in the protein structure (inaccessible to the solvent), and the reduction of such disulfide bonds often requires denaturation of the protein (high temperature heating or addition of denaturant, such as 6 M guanidine hydrochloride, 8 M urea or 1% SDS). On the contrary, according to the different reduction speed of disulfide bond in the presence of DTT, the depth of its embedding can be judged.

The usage and dosage of biochemical reagents are mainly based on the customer's experimental purpose and the experimental methods in the existing literature or books, and the following uses are for reference only.

1. Used in biochemical studies as a sulfhydryl protector; specific inhibitor of enzymes; determination of enzyme activity.

2. Preparation of SDS-PAGE buffer: Dissolve 3.09 g of DTT with 20 ml of 0.01 mol/L sodium acetate solution (pH5.2), filter and sterilize, then dispense into 1 ml portions and store at -20.

3. Inhibitory effect of dithiothreitol (DTT) eye drops on selenium cataracts (simulated senile cataracts) in SD rats: 25 mmol/L of DTT, 1 drop per eye per time (about 40 μl), for 3 weeks.

4. Dithiothreitol supplementation attenuates hepatic and renal injury in bile duct ligated mice: a potential application for cholestasis-related complications: bile duct ligated (BDL) mice were supplemented with DTT-containing drinking water (0.25% and 1% w:v) for 14 days.

Note

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