Products
Pathological Reagents | Consumables
Morphology Staining Solution
HE Stain
SKU :
Quantity
หมวดหมู่ : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
แบรนด์ : Servicebio
Share
Product Information
Product Name | Cat. No. | Spec. |
Eosin Y Solution (Alcoholic) | G1001-100ML | 100 mL |
G1001-500ML | 500 mL |
Description
Eosin Y is an acidic dye used in histology for staining cell plasma. The basic principle is that eosin dissociates into negatively charged anions in water, which can bind to the cationic amino groups of proteins in the cytoplasm and give the cytoplasm a red color.
The molecular weight of Eosin Y used in this product is 649.89, and the concentration is 0.05%. It is mostly used with Hematoxylin staining solution (G1004) for staining tissues or cells, and is used to show the cell structure. After staining, the cell plasma shows different shades of pink to peach color. Compared with G1002 Eosin Y Solution (Aqueous), the tissue or cell can be directly dehydrated by anhydrous ethanol, transparent and sealed by xylene after staining with this product without washing, and the cytoplasm of the tissue or cell is more deeply colored.
Storage and Handling Conditions
Store and transport at room temperature. Valid for 18 months.
Pre-experiment preparation
Provide your own xylene, gradient ethanol, anhydrous ethanol, and neutral gum.
Usage
1. Sample pre-processing
1) For paraffin sections: sections were sequentially deparaffinized by xylene for 10 min, replaced by fresh xylene for 10 min, anhydrous ethanol for 5 min, fresh anhydrous ethanol for 5 min, 90% ethanol for 5 min, 75% ethanol for 5 min, and washed in tap water.
2) For frozen sections: frozen sections stored at -20°C should be allowed to stand for 5-10 min to return to room temperature.
2. Eosin Y stain
The above treated sections were sequentially entered into 85% ethanol for 3-5 min, 95% ethanol for 3-5 min, and then into Eosin Y Solution (Aqueous) for 4-5 min, and then sequentially dehydrated by three times of anhydrous ethanol, each time for 4-5 min; the sections were entered into xylene for 5 min for transparency, and then replaced with fresh xylene for another 5 min for transparency.Finally, the slices were sealed with neutral tree glue.
Note: If used for restaining after staining such as immunohistochemistry, please perform Eosin Y staining after the other staining is completed.
Note
1. The purity of anhydrous ethanol used for dehydration should be greater than 99.9%. If ethanol contains water, Eosin Y will fade and cause uneven staining
2. Eosin Y solution (alcoholic) can be used repeatedly for several times, each 100 mL of Staining Solution can be used to stain approximately 150 sections. It is recommended to replace the new dye when the tissue coloring is obviously shallow
3. Please wear lab coat and disposable gloves during operation.
For Research Use Only!
