Malachite Green ELISA Test Kit

Brand: FSTest
CatNO: DR32.5074.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for quantitative detection of malachite green (MG) and its metabolite leucomalachite green (LMG) in seafood samples, ensuring food safety and regulatory compliance.

FSTest Malachite Green ELISA Test Kit – Ultra-Sensitive Detection for Banned Aquaculture Chemicals
Introduction:
The FSTest Malachite Green ELISA Test Kit is a high-precision immunoassay designed for quantitative detection of malachite green (MG) and its metabolite leucomalachite green (LMG) in seafood samples.    With an exceptional 0.15ppb detection limit, this kit enforces compliance with global bans on these carcinogenic aquaculture chemicals.

Key Features & Benefits:
  Ultra-Sensitive Detection - 0.15ppb LOD (0.01ppb LOQ) meets strictest regulatory requirements
  Dual-Analyte Coverage - Detects both MG (100%) and LMG (20%)
  Regulatory Compliance - Supports enforcement of global MG bans in aquaculture
  Specialized Seafood Testing - Optimized for fish and shrimp matrices
  High-Throughput Format - 96-well design for efficient batch processing
  Validated Performance - ≤10% CV ensures reproducible results

Applications:
Seafood safety monitoring (fish, shrimp)
Aquaculture product export testing
Food import compliance verification
Regulatory enforcement programs

Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to MG concentration) against a 6-point standard curve (0-0.08ppb).

How to Use:

1. Sample Preparation:
   Homogenize seafood samples
   Extract with acetonitrile/dichloromethane
   Purify with neutral alumina
   Oxidize with specialized reagents (fish: 30min; shrimp: 30min)
   
   
2. Test Procedure:
   Prepare fresh standard series (0-0.08ppb)
   Add 100μL antibody solution, incubate 10min
   Add 100μL sample/standard, incubate 30min
   Add enzyme conjugate, incubate 15min
   Add substrate, incubate 15min
   Add stop solution
   Read at 450nm
   
   
3. Result Interpretation:
   Compare to standard curve
   Apply 15× dilution factor

Why Choose FSTest Malachite Green ELISA Kit?
  Banned Substance Focus - Specifically designed for prohibited aquaculture chemicals
  Unmatched Sensitivity - Lowest detection limits in the industry
  Matrix-Specific Protocols - Different oxidation treatments for fish vs shrimp
  Regulatory Ready - Complies with EU/China/USFDA zero-tolerance policies
  Cost-Efficient - 96 tests/kit reduces per-sample cost

Technical Specifications:
Detection Limit: 0.15ppb
LOQ: 0.01ppb
Accuracy: 70-130% recovery
Cross-Reactivity: MG (100%), LMG (20%)
Format: 96-well microplate
Storage: 2-8°C (12 months)

User manual

• DR32.5074.5 Malachite Green ELISA Test Kit User Manual

https://www.fstestcorp.com/products-detail/id-173.html