Plant SafraninO-Fast Green Stain, 100 mL×2 (for Plant sample staining)

Product Information

Description

Safranin, also known as Safranin O, is an alkaline dye that shows lignified, corked, and keratinized tissue in vascular plants. Fast green is an acid dye that can display cellulose cell tissue containing plasma. The combination of saffron and fast green staining is widely used in animal and plant tissue and cell staining.

This product is suitable for the tissue section staining of roots, stems and leaves of plants. The main components are as follows: The effective component concentration of plant saffron staining solution is 1%, and the effective component concentration of plant fast green staining solution is 0.5%. After staining with this product, plant ducts, xylem, lignified cell walls and nuclei were red, sieve tubes, parenchyma cells, cell walls containing cellulose and cytoplasm were green.

Storage and Handling Conditions

Store and transport at room temperature; It is valid for 12 months.

Component

Assay Protocol

1. The paraffin sections are dewaxed to water.

2. Saffron staining: the sections were stamped with plant saffron staining solution and soaked for 2 min. The excess dye was quickly washed off with tap water. Then the sections were rapidly differentiated into 50%, 70% and 80% ethanol, 3-5 s each time.

3. Fast green staining: The sections are stained with plant fast green staining solution for about 6-30 s (it can be adjusted according to the thickness of the sections and the degree of staining), and the sections are removed and slightly filtered to dry the staining solution. The sections were rapidly dehydrated with absolute ethanol three times, 5-10 s each time.

4. Transparent sealing: Sections were transparent through xylene for 5 min, and then sealed with neutral gum.

Note: Prepare gradient ethanol, xylene, neutral gum, etc.

Note:

1. Paraffin sections should be fully dewaxed to avoid affecting the staining effect.

2. The washing time after saffron dyeing should not be too long, otherwise the saffron is easy to fade.

3. This product can be reused several times. After use, it is sealed and stored to prevent volatile active ingredients. Each set of staining solution can be used for staining (dip-staining) approximately 500 sections. When the tissue coloring is significantly light, it is recommended to replace a new dye solution.

4. Wear a lab coat and disposable gloves during operation.

For Research Use Only!

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