ISH Fixative Solution(animal) , 500 mL

Product Information

Description

In histopathology, tissues must be fixed in a timely and effective manner before they can be subsequently embedding and sectioning. The role of tissue fixation is to maintain the inherent form and structure of tissues and cells, prevent bacterial corrosion and autolysis of tissue cells, preserve the inherent substances in cells, coagulate intracellular proteins, minimize or terminate the reaction of endogenous and exogenous enzymes, so that cells or tissues basically remain the same as the substances in life, and also make tissues harden, which is convenient for tissue cutting. For some infectious specimens, it can prevent the spread of disease; Keep rough specimens. Finally, fixation enhances the effect of staining.

The main component of this product is 4% paraformaldehyde, with 0.1 M phosphoric acid buffer as solvent, pH 7.0-7.5@25 °C, treated with DEPC, effectively inactivate RNase in solution, specially used to fix samples that need to be tested for subsequent nucleic acid in situ hybridization.

Storage and Handling Conditions

Normal temperature transportation; Store in a cool, dry place, away from direct sunlight, valid for 24 months.

Assay Protocol

Freshly taken tissue specimens are quickly immersed in this product, the ratio of fixative solution to tissue is not less than 1:10, tissue blocks should not be too thick, 5 mm is best, the fixation time is generally 1-4 h/mm, or fixation can be carried out for 24 h for follow-up operations, large specimens can appropriately extend the fixation time. In vitro cultured cells were fixed within 15 minutes.

Note:

1. Use the product in a well-ventilated environment with proper protection to avoid inhalation.

2. In order to ensure that the fixation solution fully penetrates the tissue, the thickness of the sampling tissue should not exceed 5 mm, and the amount of fixation solution should be sufficient. If the volume is not large enough, the fixation solution can be replaced 2-3 times during fixation.

3. Please tighten the cap in time after use to prevent volatile active ingredients.

4. For your safety and health, please wear a lab coat and disposable gloves when operating. 

For Research Use Only!

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