Ultrafiltration Spin Columns

Biosharp ultrafiltration spin columns can quickly and effectively concentrate and purify biological sample. The unique vertical design and maximized filtration area can provide rapid sample processing and a high sample recovery rate (typically greater than 90% of dilute initial solution), while maintaining a mild concentration environment to preserve the activity and conformation of biological samples. Vertical design minimizes solute polarization and subsequent membrane fouling. The physical stop point in the filtration device prevents the sample from drying out and suffering losses caused by excessive rotation of the filter. Ultrafiltration molecular weight cut-off (MWCO) filter device uses PES membranes, which have a very low binding rate for proteins and nucleic acids. It is commonly used to concentrate small peptides, oligonucleotides, nucleic acids, enzymes, antibodies, and other similar macromolecules.

Features：

• Material: polypropylene (PP) cap and tube, polycarbonate (PC) filter device, polyethersulfone
  (PES) membrane
  
• Effective filtration area: 7.2cm2，Retention volume: ≤30μL
  
• Large filtration area and vertical membrane design enable fast sample processing, provide a
  high sample recovery rate
  
• Low binding rate of biomolecules and high chemical compatibility
  
• Anti-drying lock design prevents sample from being damaged by excessive centrifugation.
  Lock volume: 300 μL
  
• Maximum centrifugal force: swing-bucket rotor 4000 xg (3000 xg for 100KD), fixed-angle rotor
  5000 xg (3000 xg for 100KD)
  
• Multiple pore size specifications, meet different requirements for molecular weight cut-off
  (MWCO)

Applications：

• Concentrating and desalting proteins and nucleic acids
  
• Buffer exchange or desalting of chromatographic components
  
• Harvesting biomolecules from the culture medium
  
• Concentrating or separating viruses
  
• Crude separation of biomolecule mixtures
  
• Removing debris and particles from cell lysates
  
• Removing primers, adapters, or molecular markers from the reaction mixture

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