Gene Service
Gene synthesis service synthesizes genes according to the customer’s gene sequence order. It is widely used in areas where recombinant DNA is studied, such as vaccine manufacturing, gene treatment and character expression.
Gene Synthesis
Order form DOWLOAD HERE
Macrogen’s gene synthesis service provides synthesis services for genes in a plasmid DNA state with a free cloning service, as well as DNA sequencing. Customers can use a wider range of synthesis services via the mutagenesis service. Moreover, a 100% sequence is guaranteed based on ABI 3730xl sequencing equipment and convenient ordering and inquiries for quotations are possible through Laboratory Information Management System (LIMS).
Features
- Free cloning service (Macrogen standard vector)
- 100% sequence guaranteed using ABI 3730xl
- Mutagenesis service available
- Provided as plasmid DNA
- Free sequencing provided
- Quotation inquiries and orders possible using Laboratory Information Management System (LIMS)
Macrogen Standard Vector
Mutagenesis Service
Macrogen offers a variety of mutagenesis services such as introducing point mutations, insertions, or deletions into your gene of interest.
Sub Cloning Service
Sub-cloning the PCR product using a customer-designated vector after specific restriction enzymes treatment. Sub-cloning the template DNA and primer using a customer-designated vector after PCR.
Work Flow
Oligo Synthesis Gene Assembly Cloning DNA Sequencing & Quality Check Lyophilization Shipping
Gene in Vector
Order form DOWLOAD HERE
Service information DOWLOAD HERE
Bio Basic’s most popular gene service. Gene is synthesized as designed, then is either inserted into one of our vectors, or into a custom vector you provide.
Features
- Free Gene Optimization.
- Free cloning in standard vector.
- Bulk Order Subcloning – Please inquire
- 2-4ug of lyophilized gene in vector.
- Genes are fully cloned and 100% sequence verified.
- No hidden costs or “set-up” charges.
| Gene Length | Price* (THB) |
| 0 – 435 bp | 5,900 |
| 436 bp – 9 kb | 14-18 /bp |
| +10 kb | ติดต่อฝ่ายขาย |
| Gene Optimization | |
| Subcloning in standard vector | |
| Custom subcloning | 6,000 /gene |
Standard vectors (Free)
| pUC18 | pUC19 | pUC57 | pBR322 | pUCm-T | pUC-SP |
| pBluescript II KS+ | pBluescript II SK+ | pBluescript II SK- | pUC57-Kan | pUC57m (No NdeI) | pUC57 (no Bsal) |
Specialty vectors
| pIRES2-EGFP | pET-3a | pET-15b | pET-20b(+) | pET-22b(+) | pET-28a(+) |
| pET-30a(+) | pET-47b(+) | pCold II | pET-9a | pETDuet-1 | pET-21a(+) |
| pET-24a(+) | pET-28b(+) | pET-32a(+) | pET-49b(+) | pmirGLO | pET-11a |
| pET-19b | pET-21b(+) | pET-24b(+) | pET-29b(+) | pET-32b(+) | pET-50b(+) |
| pET-48b(+) | pEGFP-C1 | pGEX-4T-1 | pGL3-promoter | pACYCDuet-1 | pGL4.17[luc2/ Neo] |
| pET-45b(+) | pEGFP-C3 | pGEX-6P-1 | pQE-30 | pCDFDuet-1 | pGL3-Basic |
| pcDNA3.1 (+) | pGL4.19[luc2CP Neo] | pQE-60 |
Mutagenesis
Site-directed mutagenesis of a sequence to make specific, targeted changes to double stranded DNA.
Features
- Mutations can be introduced at any site, as well as on large constructs ( 8 kb)*.
- 2-4ug of lyophilized gene in vector.
- Final gene are 100% sequence verified.
- No hidden costs or “set-up” charges.
*: Template can either be submitted to us, or synthesized by us.
One mutation = any combination of base changes within a frame of 10 bases.
| Gene Length | Price* (THB) | |
| 1 point mutation | Additional mutations | |
| 1-2 kb | 6,000 | 2,900 /mutation |
* ราคายังไม่รวม vat 7%
หมายเหตุ รอบการสั่งสังเคราะห์ทุกวันพฤหัสของสัปดาห์ ระยะเวลาได้รับสินค้าไม่เกิน 7-14 วัน