Geneaid™ Maxi Plasmid Kit (Endotoxin Free)

DNA Extraction/Plasmid DNA PurificationThe Geneaid™ Maxi Plasmid Kit Endotoxin Free was designed for plasmid DNA purification and endotoxin removal using pre-packed anion-exchange resin maxiprep columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. This endotoxin free plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and the contaminants are removed with the PMC Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 100 minutes without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun.

DESCRIPTION
Specifications (Cat. # PME10, PME25)

• High Yield: 1.0 mg of pure transfection grade plasmid DNA from 300 ml cultured bacterial cells
  
• Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
  
• Large Sample Volume: 200-1600 ml of cultured bacterial cells
  
• Format: plasmid maxiprep anion-exchange resin column
  
• Elution Volume: 500 µl - 2 ml
  
• Operation time: within 100 minutes
  
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS
Transfection, Sequencing, Ligation, PCR, In-vitro Transcription Microinjection, Restriction Enzyme Digestion, Gene Gun

COMPONENTS

• PM1, PM2, PM3 Buffer
  
• TrueBlue Lysis Buffer
  
• PER Buffer
  
• PEQ Buffer
  
• PMC Buffer
  
• PEL Buffer
  
• RNase A
  
• Plasmid Maxi Columns

QUALITY CONTROL
The quality of the Geneaid™ Maxi Plasmid Kit (Endotoxin Free) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 300 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is analyzed by electrophoresis.

DOCUMENTS

Endotoxin Free Maxi Kit

GeneaidTM Maxi Plasmid Kit EF Quick Protocol

GeneaidTM Plasmid Maxi Kit 无内毒素质粒大提试剂盒

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