SKU : G1074-100ML
Categories : Biochemicals ,  1. Chemical and Reagents ,  Buffers ,  Servicebio , 
Brand : Servicebio
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Product Information
Product Name | Cat.No. | Spec. |
TTC Staining Solution(0.1%,seeds) | G1074-100ML | 100 mL |
Description
TTC (2, 3, 5-triphenyltetrazolium chloride), a lipid-soluble light-sensitive complex, can be used to detect seed viability or to detect ischemic infarction in mammalian tissues. The basic principle is that TTC is a proton acceptor of pyridine-nucleoside structural enzyme system in respiratory chain, and can react with dehydrogenase in normal tissues to form water insoluble red Triphenylformazan (TTF) compounds. Normal tissues and viable seeds are rich in dehydrogenase, which can react with TTC and appear red. If the tissue dies from ischemia or seed viability declines, it cannot react with TTC, and the tissue cannot be stained or the staining is very light. The degree of tissue ischemia infarction or seed vigor can be determined according to the staining site and depth.
The TTC concentration of this product is 0.1%, which is suitable for plant seed vigor staining. After staining, viable seeds appear red, while inactive seeds appear white or yellow.
Storage and Handling Conditions
Wet ice transportation; Store at 4 away from light, valid for 6 months.
Component
Component | G1074-100ML |
TTC Staining Solution(0.1%,seeds) | 100 mL |
Product Manual |
Assay Protocol
Sample pretreatment: Soak seeds in water at 30-35 for 2-6 h or longer to make them fully imbibe.
Dyeing steps
1. TTC Staining Solution was preheated at 37 for 30 min.
2. Peel off the seed shell or seed coat, cut longitudinally into two lobes, and put into a clean centrifuge tube. TTC staining (seeds) was added to soak the samples, and the samples were stained in a 37 water bath for 30-60 min under light, during which the seed coloring was observed every 15 min.
3. When the seeds appear red and no longer become dark, the dye solution can be poured out, and the fixation solution (G1101 recommended) is slowly added into the container to soak the tissue to terminate the staining reaction. Samples should be stored away from light.
4. Remove the tissue before photographing, blot the fixation solution with filter paper, and photograph with a camera as needed.
Note:
1. The stained samples are easy to fade, so photos should be taken as soon as possible.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
For Research Use Only!