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ปริมาณสินค้า (Quantity)
หมวดหมู่ : 2. Cell & Molecular Biology ,  DNA / RNA Extraction Kit ,  Geneaid (Taiwan) , 
แบรนด์ : Geneaid (Taiwan)
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RNA Extraction/Tri RNAGENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting. GENEzol™ Reagent is a cost effective alternative for efficient total RNA extraction.
DESCRIPTION
Advantages (Cat. # GZR050, GZR100, GZR200)
APPLICATIONS
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
COMPONENTS
GENEzol™ Reagent
QUALITY CONTROL
GENEzol™ Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. RNA from a 1 ml human blood sample is extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.
GENEzol™ Reagent Comparison Data
Figure 1 .Bacterial RNA was extracted from 5 x 108 E. coli cells using our top competitor's TRI reagent and GENEzol™ Reagent. The E. coli cells were incubated with lysozyme for 5 minutes at room temperature. 1 ml of reagent was then added to the cell lysate. The RNA pellets were dissolved in 50 μl of RNase-free water.
TRI Reagent = 1, 2
GENEzol™ Reagent = 3, 4
GENEzol™ Reagent Functional Test Data
Figure 2 .RNA from a 1 ml human blood sample was extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder
GENEzol™ Reagent real-time PCR Data
RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ TriRNA Pure Kit (red line).
RNA standard (107 copy/ml) (light green line)
RNA standard (106 copy/ml) (dark green line)
RNA standard (105 copy/ml) (light blue line)
RNA standard (104 copy/ml) (dark blue line)
Figure 3 .Quantitative analysis of human beta globin mRNA extracted by GENEzol™ using Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.
DOCUMENTS