GENEzol™ Reagent

RNA Extraction/Tri RNAGENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting. GENEzol™ Reagent is a cost effective alternative for efficient total RNA extraction.

DESCRIPTION
Advantages (Cat. # GZR050, GZR100, GZR200)

• Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
  
• Sample Volume: up to 300 μl of blood, buffy coat, serum, plasma, up to 5 x
• 106 cultured cells, 50-100 mg of tissue
  
• A cost effective, scalable solution
  
• Format: phenol, chlorform, guanidine isothiocyanate
  
• Storage: dry at 2ºC to 25ºC

APPLICATIONS
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS
GENEzol™ Reagent
QUALITY CONTROL
GENEzol™ Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. RNA from a 1 ml human blood sample is extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.
GENEzol™ Reagent Comparison Data

Figure 1 .Bacterial RNA was extracted from 5 x 108 E. coli cells using our top competitor's TRI reagent and GENEzol™ Reagent. The E. coli cells were incubated with lysozyme for 5 minutes at room temperature. 1 ml of reagent was then added to the cell lysate. The RNA pellets were dissolved in 50 μl of RNase-free water.

TRI Reagent = 1, 2
GENEzol™ Reagent = 3, 4

GENEzol™ Reagent Functional Test Data

Figure 2 .RNA from a 1 ml human blood sample was extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder

GENEzol™ Reagent real-time PCR Data

RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ TriRNA Pure Kit (red line).
RNA standard (107 copy/ml) (light green line)
RNA standard (106 copy/ml) (dark green line)
RNA standard (105 copy/ml) (light blue line)
RNA standard (104 copy/ml) (dark blue line)

Figure 3 .Quantitative analysis of human beta globin mRNA extracted by GENEzol™ using Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.

DOCUMENTS

GENEzol™ Reagent

https://geneaid.com/product.php?act=view&id=277