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| mRNA sequencing is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of gene expression in the sample.
With its wide-ranging applications, this cutting-edge tool unveils intricate gene expression profiles, gene structures, and molecular mechanisms associated with diverse biological processes.
This technique is widely adopted in research, clinical diagnostics, and drug development, since it offers insights into the intricacies of cellular dynamics and genetic regulation, providing a sturdy base to spark curiosity about its potential on different fields.
Platforms available: Illumina NovaSeq X; DNBSEQ-T7  
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Features
- Optional directional mRNA library preparation to enable obtaining strand-specific sequencing data
- Different BI analysis cover all our clients needs |
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Advantages
- Extensive Expertise: We have processed over 600,000 samples, spanning diverse sample types such as cell cultures, tissues, and body fluids. We bring the wealth of expertise to every project.
- Rigorous Quality Control: We implement core control points across all stages, from sample preparation to library preparation, sequencing and bioinformatics. Our meticulous monitoring ensures the delivery of consistently high-quality results.
- Comprehensive Annotation: We use multiple databases to functionally annotate the Differentially Expressed Genes (DEGs) and perform corresponding enrichment analyses. This comprehensive approach provides insights into the cellular and molecular processes underlying the transcriptome response, ensuring you get all the information possible about your experiment’s data.
- Post-Sales Support: We understand how important is to be present, that’s why our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results. |
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Sample Requirements and Delivery
| Library | Sequencing strategy
| Data recommended | Quality Control | | Poly A enriched | Illumina PE150
DNBSEQ-T7 | 6 -10 Gb | Q30≥85% |
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Sample Requirements:
Nucleotides:
Mimimum Sample Requirements:
| Conc.(ng/μl) | Amount (μg) | Purity | Integrity | Standard Library
≥ 10
| ≥ 0.2 | OD260/280=1.7-2.5
OD260/230=0.5-2.5
Limited or no protein or DNA contamination shown on gel. | For plants: RIN≥4.0;
For animals: RIN≥4.5;
5.0≥28S/18S≥1.0;
limited or no baseline elevation
| Directional Library
≥ 10
| ≥ 0.2 | OD260/280=1.7-2.5
OD260/230=0.5-2.5
Limited or no protein or DNA contamination shown on gel. | For plants: RIN≥4.0;
For animals: RIN≥4.5;
5.0≥28S/18S≥1.0;
limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells | Root, Stem or Petal: 450 mg
Leaf or Seed: 300 mg
Fruit: 1.2 g | Heart or Intestine: 300 mg
Viscera or Brain: 240 mg
Muscle: 450 mg
Bones, Hair or Skin: 1g | Insects: 6g
Crustacea: 300 mg | 1 tube | 106 cells |
Recommended Sample Delivery Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3... ...
Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2. RNAs table tubes: RNA samples can be dried in RNA stabilization tube (e.g. RNAstable®) and shipped in room temperature. |
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Service Work Flow
 |  |  |  |  |  |  | | Experiment design | Sample delivery | RNA extraction | Library construction | Sequencing | Data analysis | After-sale services |
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