Exosomes are small vesicles secreted by cells, typically ranging in diameter from 30 to 100 nanometers. These vesicles contain various RNA. Among the RNA types in exosomes, the most common and extensively studied is microRNA (miRNA). miRNA is a class of non-coding small RNAs approximately 18-25 nucleotides in length. They mediate post-transcriptional gene silencing by binding to the 3′ untranslated region (3′ UTR) of target mRNAs, thereby regulating gene expression. For example, exosomes secreted by certain tumor cells contain specific miRNAs, such as miR-126 and miR-92a. These miRNAs can influence gene expression in recipient cells and promote tumor angiogenesis (Tomohiro Umezu, et al., Oncogene, 2012).

  Features

- Exosomal total RNA is used as input material. During purification, miRNA Beads are employed to isolate miRNA.
- Bioinformatic analysis centered around miRNA prediction and their targets.

  Service Advantages

- Comprehensive bioinformatics analysis: Enabling the identification of both known and novel miRNAs, identification of miRNAs targets, and corresponding functional annotation and enrichment with multiple databases (KEGG, GO).

- Rigorous Quality Control: We implement core control points across all stages, from sample and library preparation to sequencing and bioinformatics. This meticulous monitoring ensures the delivery of consistently high-quality results.

- Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.

  Sample Requirements and Delivery

  Sample Requirements:
Nucleotides:

Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3.

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2. RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped at room temperature.

  Service Work Flow

Experiment design	Sample delivery	RNA extraction	Library construction	Sequencing	Data analysis	After-sale services

-  Raw data quality control
-  sRNA classification
-  Alignment to a reference genome
-  Identification of known and novel miRNA
-  Differential miRNA expression analysis
-  Functional annotation of miRNA targets

Identification of miRNA: structure and depth

Differential expression of miRNA – hiearchical clustering

Functional annotation of target of differentially expressed miRNAs