Oligonucleotide Synthesis
บริการสังเคราะห์ไพร์เมอร์ (Primer Synthesis)
Macrogen provides the best oligo synthesis service with the latest equipment and an automated system.
High-quality oligos are produced via a purification process by removing impurities and
oligos that are nonspecifically bound. The quality of all oligos is controlled by MALDI-TOF mass spectrometry.
Features
· Scales from 0.05 umole to 1.0 umole
· Quick delivery by express courier (using FedEx / DHL International shipping)
· Accurate concentration provided by the Liquid Handling System
· Orders, progress of synthesis, delivery, and results of synthesis can be confirmed
by Laboratory Information Management System (LIMS)
Refined Quality
MOPC
(Macrogen Oligonucleotide Purification Cartridge)
| HPLC | PAGE |
- Available for both standard oligos and some modified oligos - Purity of ≥ 85 % (LC Analysis) - Available for free as a default for all oligos
| - Available for all standard oligos and modified oligos - Recommended for oligos of 50 mer or less - Purity of ≥ 90 % (LC Analysis) | - Available for all standard oligos and modified oligos - Recommended for oligos of 50 mer or less - Purity of ≥ 95 % (LC Analysis) |
100% Quality Control(OD/MALDI-TOF)
· Standard Oligo : Can be synthesized for 3 ~ 130 mer(However, it is not provided with MALDI Q/C after not more than 5mer or 90mer)
· Modified Oligo : Up to 60 mer is recommended(Inquire for oligos of 60 mer or more)
Guaranteed Yield(OD) | Synthesis Scale(25mer 기준) |
0.05 umole | 0.2 umole | 1 umole |
MOPC(Standard Oligo) | 6 | 8 | 20 |
MOPC(Modified Oligo) | - | 5 | 10 |
PAGE | 2 | 4 | 8 |
HPLC | 2 | 4 | 8 |
Release and delivery of synthesized oligos occur after OD measurement
and MALDI-TOF MS analysis to ensure quality. The result report is shipped with the ordered oligos.
Use MALDI-TOF mass spectrometry equipment to accurately analyze Oligo and identify Oligo,
which includes N-1, N-2 mers, etc.