SYNONYMS
L-dopachrome tautomerase (EC:5.3.3.12), DCT, DT, L-dopachrome Delta-isomerase Tyrosinase-related protein 2, TRP-2, TRP2, TYRP2.
INTRODUCTION
Dopachrome Tautomerase also known as DCT, belongs to the tyrosinase family. DCT takes a significant part in the synthesis of the melanin pigment including tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1). Which are highly involved in eumelanin synthesis. The change among the eumelanin and the pheomelanin pathways is assumed to depend on the presence of cysteine. Hence, in the lack of cysteine, dopaquinone, the product of tyrosinase action, is transformed to cyclodopa (leucodopachrome) and afterwards to dopachrome (and DOPA).
DESCRIPTION
DCT Human Recombinant produced in Sf9 Insect cell is a single, non-glycosylated polypeptide chain containing 455 amino acids (24-472aa.a) and having a molecular mass of 52.1kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions).
DCT is fused to a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
