SKU : G3421-200U
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
Inorganic Pyrophosphatase (Thermostable) | G3421-200U | 200 U |
Product Introduction
Product Description: | Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 +H2O+PPase 2HPO4-2). The enzyme requires a divalent metal cation, with Mg2+ conferring the highest activity. This product exerts its effect at 16-37, showing the strongest effect at 25. The Inactivation temperature is above 65. |
Applications: | l High yield RNA synthesis by in vitro transcription. l DNA polymerization reactions: preventing accumulation of pyrophosphate. l Removal of contaminant PPi in reagents used for SNP genotyping by methods based on the detection of pyrophosphate. |
Source: | An E. coli strain carrying a plasmid encoding a pyrophosphatase from the extreme thermophile Thermococcus litoralis. |
Purity: | 95% by SDS-PAGE |
Endogenous nucleic acid | ＜1 pg/μL by qPCR |
Concentration: | 2 U/μL |
Definition of Activity Unit: | One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 100 mM Tris-HCl [pH 8.0], 2 mM MgCl2, 2 mM PPi, reaction volume of 0.5 ml). |
Storage (Dilution) Buffer: | 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0. |
Inactivation or inhibition | l Inhibitors: imidodiphosphate, α ω glycol disphosphates, methanedial diphosphate, 1,2-ethanedial diphosphate. · l Inactivation by heating is not complete, reliably removed by spin column or phenol/chloroform extraction. |
Storage Conditions: | Store at 20°C up to 12 months. |
Product Contents
Component Number | Component | G3421-200U |
G3421-1 | Inorganic Pyrophosphatase (Thermostable) | 100 μL |
Manual | One copy | |
Assay Protocol / Procedures
The Inorganic Pyrophosphatase (Thermostable) can be directly added into the reaction system.
Working concentration of the enzyme range from 0.05 to 1 U/mL.
Note
l Enzymes should be placed on ice when used, and stored at -20 immediately after use. It is recommended to store separately.
1. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
