Prussian blue Stain, 100 mL×3 (for Pigment staining)

Product Information

Description

Prussian blue staining, also known as iron staining, hemosiderin staining or Perls staining, shows primarily trivalent iron and is a traditional method for showing hemosiderin or ferric ion in tissue. The principle of dyeing is to separate ferric ions from proteins by dilute hydrochloric acid using potassium ferrocyanide. Any ferric ion in the tissue combines with ferrocyanide and results in the formation of an insoluble blue pigment called ferric ferrocyanide or prussian blue.

This product is a set in which solution A is low concentration hydrochloric acid aqueous, solution B is 2% potassium ferrocyanide solution, C is 0.1% nuclear fast red solution. After staining, the ferric ion or hemosiderin in the tissue cells is blue, and the nucleus is red.

Storage and Handling Conditions

Room temperature, valid for 12 months.

Component

Pre-experiment preparation

1. Preparation of Prussian blue staining working solution: Mix Prussian blue staining solution A and Prussian blue staining solution B in equal volume. Prepare the solution before use and use it now.

2. Provide your own gradient of ethanol, xylene, and neutral gum.

Assay Protocol

1. Paraffin sections were dewaxed to water.

2. Drops of Prussian blue staining working solution were added to completely cover the tissues and stained at room temperature for 1 h. The staining solution was decanted and the sections were immersed into pure water and washed 3 times.

3. Drops of Prussian blue staining solution C were added to cover the tissues, stained for 3-5 min at room temperature, and washed with tap water until the running water of the section was colorless.

4. Sections were sequentially dehydrated by anhydrous ethanol three times for 5 min each, then transparent by xylene for 5 min, and then sealed with neutral gum.

Note

1. The tissue should be fixed with neutral formaldehyde solution, general purpose tissue fixative (G1101) manufactured by our company is recommended.

2. Make sure the container is clean during the operation, preferably glass, and avoid iron containers.

3. Sections were washed with distilled water before dyeing to prevent false positive reaction caused by interference of iron ions in tap water.

4. Each set of staining solution can be used to stain approximately 100 sections. Replace the stain with a new one when the tissue or cell coloring is significantly lighter or abnormal..

5. Please wear lab coat and disposable gloves during operation.

For Research Use Only!

https://www.servicebio.com/goodsdetail?id=1773