Quinolones ELISA Test Kit

Brand: FSTest
CatNO: DR32.5071.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for quantitative detection of 10 major quinolone antibiotics in animal tissues, seafood, eggs, honey, and milk, ensuring food safety and regulatory compliance.

FSTest Quinolones ELISA Test Kit – Comprehensive Antibiotic Residue Detection for Food Safety
Introduction:
The FSTest Quinolones ELISA Test Kit is a high-performance immunoassay designed for quantitative detection of 10 major quinolone antibiotics in animal tissues, seafood, eggs, honey, and milk.   With a 0.5ppb detection limit for most matrices (5ppb for milk), this kit provides reliable monitoring of these widely-used veterinary antibiotics to ensure food safety compliance.

Key Features & Benefits:
  Broad-Spectrum Detection - Covers 10 quinolones including enrofloxacin (100%), ciprofloxacin (110%), and norfloxacin (119%)
  Multi-Matrix Validation - Certified for 5 sample types with matrix-specific protocols
  Regulatory-Grade Sensitivity - 0.5ppb LOD meets global MRL requirements
  High-Throughput Format - 96-well design for efficient batch processing
  Optimized Workflow - Simplified extraction for different sample types
  Consistent Performance - ≤10% CV ensures reproducible results

Applications:
Livestock and poultry antibiotic monitoring
Aquaculture and seafood safety testing
Dairy and egg production quality control
Honey export compliance verification
Veterinary drug residue surveillance programs

Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to quinolone concentration) against a 6-point standard curve (0-12ppb).

How to Use:

1. Sample Preparation:
   Meat/Seafood/Eggs: Acetonitrile/dichloromethane extraction (3g sample)
   Honey: Dual-phase extraction
   Milk: Simple 10× dilution (100μL sample)
   
   
2. Test Procedure:
   Add 50μL sample/standard
   Add 50μL enzyme conjugate & antibody
   Incubate 30min (20-28°C)
   Wash 4-5 times
   Add substrate, incubate 30min
   Add stop solution
   Read at 450nm
   
   
3. Result Interpretation:
   Compare to standard curve (0.5-12ppb)
   Apply matrix-specific dilution:
   1× for tissue/seafood/eggs/honey
   10× for milk

Why Choose FSTest Tetracyclines ELISA Kit?
  Most Comprehensive Coverage - Detects 10 key quinolones in one test
  Matrix-Specific Protocols - Optimized methods for each sample type
  Time-Efficient - Complete analysis in<2.5 hours
  Cost-Effective - 96 tests/kit reduces per-sample cost
  Regulatory Ready - Meets EU/China/USDA requirements

Technical Specifications:
Detection Limits:
Tissue/Seafood/Eggs/Honey: 0.5ppb
Milk: 5ppb
LOQ: 0.5ppb
Accuracy: 70-130% recovery
Precision: ≤10% CV
Format: 96-well microplate
Storage: 2-8°C (12 months)

User manual

• DR32.5071.5 Quinolones ELISA Test Kit User Manual

https://www.fstestcorp.com/products-detail/id-171.html