Naegleria fowleri

Cat no. YSL-qP-EC-N.fowle-100

Human, qPCR, SinglePlex

A complete qPCR kit for the detection of Naegleria fowleri

Naegleria fowleri, also known as the 'brain-eating amoeba,' is a deadly pathogen that can cause a rare but extremely severe infection called primary amebic meningoencephalitis (PAM). This amoeba is commonly found in warm freshwater environments and enters the body through the nasal passages, leading to symptoms such as headache, fever, nausea, and altered mental status. Detection of Naegleria fowleri is crucial, especially in water sources used for recreational activities, as early diagnosis can prevent the potentially fatal outcome of PAM. This target is of particular importance in the environmental and public health sectors.

YouSeq's qPCR kit to detect Naegleria fowleri allows for highly sensitive and specific detection of this pathogen in water samples, enabling early identification and intervention to prevent infections. Rapid and accurate detection of Naegleria fowleri is essential for safeguarding public health, as prompt action can prevent outbreaks of PAM and potentially save lives. By using YouSeq's qPCR product, health authorities and environmental agencies can efficiently monitor water sources for the presence of this dangerous pathogen, reducing the risk of exposure and improving overall safety.

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Contents

• Target Specific primer/probe
  
• Endogenous control primer/probe
  
• qPCR OneStep MasterMix
  
• Positive control template
  
• DNase/RNase free water
  
• Template resuspension buffer
  
• ROX passive reference

Technical specification
Target Gene(s):Mp2Cl5 gene
Efficiency: 90-110%
Sensitivity: <100 copy detection
Run Time: Approx 1hr30 (dependent on instrumentation)
Target Pathogen: FAM labelled
Endogenous Control: VIC/HEX labelled