BioFact™ One Step RT-PCR Kit, Enzyme mixture, Reaction Buffer,5X Band Helper™, each 10mM dNTP, 500 reaction

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SKU : BR861-050

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BioFact™ OneStep RT-PCR Series is designed to save time and cost by synthesis of cDNA from RNA and amplification in a single tube at OneStep. Included BioFact™ RTase and BioFact™ H-Star Taq DNA Polymerase is mixed in the mixture as a best optimized rate to get greater efficiency and high specificity. BioFact™ OneStep RT-PCR Series is provided as three types for easy-use: standard kit (enzyme mixture, buffer, 5X Band Helper™, dNTP mixture, RNase-free water), Master Mix (master mix in 1.5ml tube) and Pre-Mix (master mix in 0.2ml 8-strip tube). 

  • Fast and convenient OneStep RT-PCR reaction in a single tube
  • Great efficiency & High specificity
  • Dual advantage of BioFact™ RTase and BioFact™ H-Star DNA Polymerase (HotStart)
  • Synthesis cDNA at 50℃
  • Vatious type ; Standard. Master Mix and Pre-mix type

FIGURES (Q,C)

DETAILED INSTRUCTIONS

FEATURES

  • RNase H : M-MLV RTase (RTase H-)
  • Temperature of synthesis cDNA : 42 - 55℃
  • Synthesis length : < 1 kb
  • Synthesis primer : Gene specific primer
  • Source : Thermus aquaticus
    (BioFact™ H-Star)
  • Amplification size: < 1 kb

APPLICATION

  • RT-PCR

STORAGE TEMPARATURE

  • -20℃ (Stable for 7 days at 25℃)

EXPIRATION DATE

  • 1 Year
    (in case of storage at -20℃)

 

http://bio-ft.com/en/pro1_2_02

 

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