HBV AG-1

TYPE
Mouse antibody Monoclonal.

INTRODUCTION
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.

CLONE
NYRHepB-1.

IMMUNOGEN
r.Hep B vaccine (BTG).

IG SUBCLASS
mouse IgG2b.

PURIFICATION METHOD
Ion exchange column.

FORMULATION
1mg/ml in PBS (after reconstitution).

SHIPPING CONDITIONS
Antibody is shipped lyophilized at ambient temperature.

STORAGE PROCEDURES
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

SOLUBILITY
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

APPLICATIONS
Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry.

TITER
By direct ELISA (against r.hepatitis vaccine), 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

SAFETY DATA SHEET
SDS

NOTE
This antibody recognizes both the AD and the AY antigens in direct ELISA and works in capture ELISA with anti-hepatitis Ag-2.

USAGE
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

https://www.prospecbio.com/hbv_ag_1_antibody