TYPE
Rat Anti Mouse Monoclonal.
INTRODUCTION
IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.
CLONE
NYRmIL-12p75.
IMMUNOGEN
r.Murine IL-12 p75.
IG SUBCLASS
Rat IgG2b.
PURIFICATION METHOD
Ion exchange.
FORMULATION
1mg/ml in PBS (after reconstitution).
SHIPPING CONDITIONS
Antibody is shipped lyophilized at ambient temperature.
STORAGE PROCEDURES
In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
SOLUBILITY
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
APPLICATIONS
Direct ELISA, Western Blot, Immuneprecipitation.
TITER
By direct ELISA, 1:5,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
SAFETY DATA SHEET
SDS
NOTE
This antibody recognizes the entire IL-12 molecule and has no reactivity to denatured p40 or p35.
USAGE
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
