SYNONYMS
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
TYPE
Rat Anti Mouse Monoclonal.
INTRODUCTION
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
CLONE
MI10-L2
IMMUNOGEN
r.Murine IL-10.
IG SUBCLASS
Rat IgM.
PURIFICATION METHOD
Boric Acid precipitation/ S-Sepharose.
FORMULATION
1mg/ml in PBS (after reconstitution)
SHIPPING CONDITIONS
Antibody is shipped lyophilized at ambient temperature.
STORAGE PROCEDURES
In lyophilized form, for long periods, store at 4ºC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20ºC.
SOLUBILITY
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
TITER
By direct ELISA, 1:1000, By western Blot, 1:2000.
SPECIFICITY
Mouse IL-10.
CROSS REACTIVITY
Does not cross-react with human IL-10.
SAFETY DATA SHEET
SDS
USAGE
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
