SYNONYMS
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
TYPE
Mouse Anti Human Monoclonal.
INTRODUCTION
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
CLONE
NYR-hIL1b.
IMMUNOGEN
r.Human IL-1b.
IG SUBCLASS
Mouse IgG2b.
PURIFICATION METHOD
Ion Exchange.
FORMULATION
1 mg/ml in PBS (after reconstitution).
SHIPPING CONDITIONS
Antibody is shipped lyophilized at ambient temperature.
STORAGE PROCEDURES
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
SOLUBILITY
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
APPLICATIONS
Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry.
NOTE
The antibody was produced in BALB/c mice.
TITER
By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). 1:2,000 dilution will completely inhibit a thymocyte co-stimulation assay using suboptimal doses of concanavalin A.
SAFETY DATA SHEET
SDS
USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
