SYNONYMS
Zeta-globin, HBAZ, HBZ2, Hemoglobin subunit zeta, Hemoglobin zeta chain, HBZ.
INTRODUCTION
Hemoglobin subunit zeta (HBZ) is part of the globin family. Hemoglobin Zeta is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life.
DESCRIPTION
HBZ Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 150 amino acids (1-142 a.a.) and having a molecular mass of 16.7 kDa. The HBZ is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.
SOURCE
Escherichia Coli.
PHYSICAL APPEARANCE
Sterile Filtered clear colorless solution.
FORMULATION
The HBZ solution contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl and 20% glycerol.
STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
AMINO ACID SEQUENCE
MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YRLEHHHHHH.
PURITY
Greater than 90% as determined by SDS-PAGE.
SAFETY DATA SHEET
SDS
USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
