SYNONYMS
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, JTK14, TIE, TIE1.
INTRODUCTION
TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.
DESCRIPTION
Soluble TIE-1 Mouse Recombinant fused with the Fc part of human IgG1 produced in CHO cells is a glycosylated disulfide-linked homodimeric polypeptide chain containing amino acids 23-749 and having a total molecular mass of 260kDa. The Mouse TIE-1/Fc monomer has a calculated molecular mass of approximately 105kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 130kDa protein in SDS-PAGE under reducing conditions.
The TIE1 Fc Chimera is purified by proprietary chromatographic techniques.
