SYNONYMS
Thymine-DNA Glycosylase, G/T Mismatch-Specific Thymine DNA Glycosylase, EC 3.2.2.29.
INTRODUCTION
Thymine-DNA glycosylase (TDG) is a member of the TDG/mug DNA glycosylase family.
TDG is a nuclear protein that fixes G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. In addition, TDG removes uracil and 5-bromouracil from mispairings with guanine. The TDG enzyme has an essential role in cellular defense against genetic mutation triggered by the spontaneous deamination of 5-methylcytosine and cytosine.
DESCRIPTION
TDG Human Recombinant produced in E. coli is a single polypeptide chain containing 433 amino acids (1-410) and having a molecular mass of 48.4 kDa.
TDG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
