SKU : enz-1035
Recombinant Firefly Luciferin 4-Monooxygenase, Active
Categories : Prospec ,
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SYNONYMS
Luciferase-like monooxygenase, LUC, EC 1.13.12.7.
INTRODUCTION
Luciferase is a general term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which involves the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is broadly used as a reporter for studying gene regulation and function, and for pharmaceutical screening.
DESCRIPTION
Luciferase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (1-311 a.a) and having a molecular mass of 38.5kDa.
Luciferase is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
