Geneaid™ DNA Isolation Kit (Bacteria), 1 ml/rxn

Reagent DNA Extraction/Reagent DNA ExtractionThe Geneaid™ DNA Isolation Kit (Bacteria) offers a simple and gentle reagent DNA precipitation method for isolating protein free, high molecular weight genomic and viral DNA from Gram (+) positive and Gram (-) negative bacteria cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

DESCRIPTION
Specifications (Cat. # GEE150, GEE1.5K, GEE1.5K+)

• Isolate high molecular weight DNA for archiving or sensitive downstream applications
  
• High yield, High quality DNA (A260/A280 = 1.8-2.0)
  
• Sample: 0.5-1.5 x 109 bacteria cells
  
• Easily scalable to satisfy larger bacteria sample volumes
  
• Minimal hands on time
  
• Convenient, simple and gentle reagent DNA precipitation method
  
• Storage: dry at room temperature (15-25°C) for up to 2 years, Lysozyme is shipped at room temperature and should be stored at -20°C for up to 1 year

APPLICATIONS
DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

COMPONENTS

• Cell Lysis Buffer
  
• Gram+ Buffer
  
• Protein Removal Buffer
  
• DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
  
• RNase A (10 mg/ml) - not included with GEE1.5K
  
• Lysozyme - not included with GEE1.5K

QUALITY CONTROL
The quality of the Geneaid™ DNA Isolation Kit (Bacteria) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 bacteria cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

DOCUMENTS

Geneaid™ DNA Isolation Kit

GeneaidTM DNA Isolation Kit (Bacteria) Quick Protocol

https://www.geneaid.com/product.php?act=view&id=250