GenepHlow™ PCR Cleanup Kit

DNA Extraction/PCR CleanupThe GenepHlow™ PCR Cleanup Kit was designed to recover, concentrate or purify DNA fragments from PCR or other enzymatic reactions. This PCR purification kit includes a pH indicator as an optional addition to the PB binding buffer to ensure optimal pH and facilitate DNA binding. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. The GenepHlow™ PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.

DESCRIPTION
Specifications (Cat. # DFC100, DFC300)

• High Efficiency: up to 95% recovery from PCR or other enzymatic reactions
  
• Only 10 minutes PCR cleanup
  
• Includes a pH Indicator for easy determination of optimal pH
  
• Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
  
• Sample Size: up to 100 μl of PCR products
  
• Broad Fragment Size Range: 100 bp–20 kb
  
• Spin Columns: glass fiber membrane optimized for PCR cleanup
  
• Elution Volume: 20-50 µl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

• PB Buffer
  
• pH Indicator
  
• 3M Sodium Acetate (pH5.0)
  
• Wash Bufffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• DFH Columns
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the GenepHlow™ PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from PCR products and analyzed by electrophoresis.

DOCUMENTS

GenepHlow™ PCR Cleanup Kit

GenepHlow™ PCR Cleanup Kit Quick Protocol

GenepHlowTM PCR Cleanup Kit 纯化回收试剂盒

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