Presto™ Soil DNA Extraction Kit

DNA Extraction/Genomic DNA PurificationThe Presto™ Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized using SL1 Lysis Buffer combined with ceramic beads in Beadbeating Tubes. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire soil DNA extraction procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

DESCRIPTION
Specifications (Cat. # SLD050, SLD100)

• Purify genomic DNA from soil within 40 minutes!
  
• Sample: 250-500 mg of soil
  
• High Yield: up to 5 µg
  
• High Quality DNA: A260/A280 = 1.7-2.0
  
• Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  
• Elution Volume: 30-100 µl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• SL1 Buffer
  
• SL2 Buffer
  
• SL3 Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• Inhibitor Removal Columns
  
• GD Columns
  
• Beadbeating Tubes Type C
  
• 2 ml Centrifuge Tubes
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the Presto™ Soil DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 250 mg soil samples. Following the purification process, a yield of more than 2 μg of genomic DNA is obtained and the A260/A280 ratio is between 1.7-2.0. The purified genomic DNA is analyzed by electrophoresis.

DOCUMENTS

Presto™ Soil DNA Extraction Kit

PrestoTM Soil DNA Extraction Kit Quick Protocol

https://www.geneaid.com/product.php?act=view&id=210