Presto™ 96 Well DNA Bacteria Advanced Kit

DNA Extraction/Genomic DNA PurificationThe Presto™ 96 Well DNA Bacteria Advanced Kit is optimized for high-throughput genomic DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. After directly adding bacteria samples into beadbeating microtubes pre-filled with zirconia/ceramic beads and lysis buffer, cells are efficiently lysed via bead beating instrument without enzymatic digestion. The cell lysate is then mixed with a binding buffer and the genomic DNA is bound by the membrane in the 96 well plate, followed by wash and elution. The entire procedure can be completed within 90 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

DESCRIPTION
Specifications (Cat. # 96GBBA04, 96GBBA10)

• 96 well bead beating microtubes pre-filled with zirconia/ceramic beads
  
• Enzymatic digestion is not required!
  
• High-throughput bacteria DNA extraction
  
• High Yield: up to 5 μg of genomic DNA per well
  
• High Purity gDNA: A260/A280 >1.9
  
• DNA Size: 20-40 kb
  
• Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 108)
  
• 96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
  
• Elution volume: 60-100 µl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

COMPONENTS

• GT Buffer
  
• PR Buffer
  
• GB Buffer
  
• W1 Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  
• Presto™ gDNA 96 Well Binding Plates
  
• Beadbeating Microtubes (Racked)
  
• Beadbeating Microtubes (8-strip)
  
• Caps for Microtubes (8-strip)
  
• 96 Deep Well Plates
  
• Adhesive Film
  
• 0.35 ml Collection Plate

QUALITY CONTROL
The quality of the Presto™ 96 Well DNA Bacteria Advanced Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (5×108) culture. 5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

DOCUMENTS

Presto™ 96 Well DNA Bacteria Advanced Kit

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