Recombinant Human KITLG Protein (His-tag)

Gene Name Synonym

KITLG, MGF, SCF

Species

Human

Accession

P21583

Source

CHO cells

SPECIFICATIONS

Amino acid sequence

Glu26-Ala190 is expressed with a 10×His tag at the C terminus

Molecular Weight

19.9 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

KIT proto‐oncogene ligand (KITLG), also known as stem cell factor (SCF), is a ligand of the c‐KIT tyrosine kinase receptor with multiple biological functions. KITLG can exist both as a transmembrane protein and a soluble protein. KITLG is a pleiotropic factor which is found in diverse cancers and is involved in cell proliferation, differentiation, and survival. KITLG is a new hallmark of type A and AB thymomas which induce a series of aberrant alteration of mRNA, miRNA and DNA methylation. This protein can augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. SCF plays an important role in the hematopoiesis during embryonic development. KITLG may be used along with other cytokines to culture HSCs and hematopoietic progenitors. In many studies we could know KITLG is perhaps another area for the exploration of thymoma with autoimmune disease.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human KITLG Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 22-30 kDa.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 5-10 ng/mL.