Recombinant Human EGF Protein (His-tag)

Gene Name Synonym

EGF, URG, HOMG4

Species

Human

Accession

P01133

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Pro23-Lys1032 is expressed with a 10×His tag at the C terminus

Molecular Weight

113.6 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

EGF is the founding member of the EGF-family of proteins. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. Members of this protein family have highly similar structural and functional characteristics. Epidermal growth factor (EGF) participates in the progress of skin proliferation, differentiation and so forth. The EGF stimulation system for growth regulation is implicated in normal and neoplastic cell proliferation. The presence of EGF in gastric cancer is correlated with the degree of gastric wall invasion and lymph node metastasis. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human EGF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using Balb/C 3T3 A31 mouse embryonic fibroblast cells. The ED50 for this effect is 5-10 ng/mL.