SKU : G2011-100ML
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
Ponceau S Staining | G2011-100ML | 100 mL |
Product Description/Introduction
Ponceau S staining solution can be used for the rapid detection of proteins on PVDF, nitrocellulose (NC) and cellulose acetate membranes. The principle is that Ponceau S is negatively charged and can bind to positively charged amino acid residues, while Ponceau S can also bind to non-polar regions of the protein, resulting in a red band. The staining of protein by Ponceau S is reversible and can be washed off with distilled water, PBS or other appropriate solution.
The main ingredient of this product is 0.2% Ponceau S.
Storage and Shipping Conditions
Ship and store at room temperature, valid for 12 months.
Product Components
Component | G2011-100ML |
Ponceau S Staining | 100 mL |
Manual | |
Assay Protocol/Procedures
1. The PVDF, nitrocellulose or cellulose acetate membranes are immersed in Ponceau S working solution, and the staining is shaken for 3-5 min or longer until clear bands appeared.
2. Rinse the stained membrane in distilled water for a few seconds to slightly remove the background color until the background is light and the protein band is clear.
3. Rinse with distilled water, PBS or other appropriate solution 2-3 times, 3-5 min each time to remove Ponceau S, and conduct the subsequent Western experiment.
Note
1. Ponceau S staining solution is a ready-to-use product and does not require dilution.
2. Ponceau S staining solution can be recycled and used repeatedly.
3. Ponceau S staining solution is not suitable for protein staining detection on nylon membrane.
4. The kit is easy to use, has a low background and high sensitivity, with a lower limit of detection of 250 ng for proteins.
5. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only.!
https://www.servicebio.com/goodsdetail?id=1815
