Antibody Stripping Buffer (Normal), 100 mL

Product Information

Product Description

Antibody Stripping Buffer is a reagent that safely and efficiently removes primary and secondary antibodies from nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes, facilitating the re-detection of chemiluminescent protein immunoblots.

Save time and effort, eliminate errors: No need to re-gel, sample and electrophoresis; save precious samples; eliminate re-sampling errors.

Quick and efficient: Incubate for 5-15 minutes at room temperature to strip antibody.

Mild formulation: Does not interfere with protein re-assay after elution.

Odorless: Thiol-free, no offensive odors.

Ready and easy to use: No dilution necessary, ready to use with open cap.

Storage and Shipping Conditions

Ship with wet ice, store at 2-8, valid for 12 months.

Components

Assay Protocol

1. Wash blot once for 5 minutes in TBST to remove the chemiluminescent substrate.

2. Pour off the TBST, add sufficient Antibody Stripping Buffer to cover the NC or PVDF membranes and incubate for 5 to 15 minutes at room temperature with gentle shaking.

3. Remove the blot from the Antibody Stripping Buffer and wash 3 times for 5 minutes each in TBST.

4. Follow-up work such as blocking and antibody incubation is performed according to the purpose of the experiment.

Note

1. This product is corrosive, take caution when using it.

2. Reuse is not recommended.

3. For your safety and health, please wear safety glasses, gloves, or protective clothing.

Table 1: Reference for antibody stripping buffer selection

For Research Use Only!

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