SKU : G2057-1L
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
10× Tris-Glycine Transfer Buffer | G2057-1L | 1 L |
Product Description
This product is a 10-fold concentrate consisting of 200 mM Tris, 1.92 M glycine, without methanol or anhydrous ethanol. Before use, take 100 mL of this product, then add 600 mL of deionized or distilled water to mix, then continue to add 200 mL of anhydrous ethanol to mix, and finally replenish the deionized or distilled water to fix the volume to 1 L. After mixing, it will be obtained 1x Tris- Glycine transfer working solution of 20 mM Tris, 192 mM glycine, 20% anhydrous ethanol, pH 8.2-8.6 at 25°C. 10× Tris-Glycine Transfer buffer is suitable for wet or semi-dry transfer of Western-Blot experiments.
Storage and Shipping Conditions
Ship and store at room temperature, valid for 12 months.
Assay Protocol
1. For conventional small to medium molecular weight proteins (25-70 kDa), the recommended conditions for transfer are 300 mA for about 30 min.
2. For conventional large molecular weight proteins, the transfer time can be extended as appropriate, with a recommended transfer time of 300 mA for 45-60 min.
3. For small molecular weight proteins below 20 kDa, it is recommended to use a smaller pore size of 0.22 μm PVDF membrane with 200 mA for 20-25 min or 300 mA for 15-20 min. The methanol content (which can be replaced by ethanol) can also be increased to 25% to enhance the fixation of small molecular proteins in the gel.
4. For proteins with particularly large molecular weight, it is recommended to extend the transfer time to 300 mA for 60-90 min or longer, add an appropriate amount of SDS to the transfer solution to the final concentration of about 0.5 to enhance the release of proteins from the gel. At the same time, due to the long transfer time, attention should be paid to the effect of the cooling module, a new cooling module can be replaced halfway if necessary.
Note
1. Please use the working solution as soon as possible after preparation. It is recommended that the reagents be diluted with purified water for laboratory use (G4701-500ML).
2. This product may have crystal precipitation in the case of low ambient temperature, this is a normal phenomenon, you can moderate heating, magnetic stirring to help dissolve, to be completely dissolved before dilution and use.
3. The normal color of this product is colorless and transparent, if it appears light brown or yellowish brown, it should be discarded promptly.
4. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
