SKU : G2145-1L
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
1× Tris-Glycine Transfer Buffer (Ready to Use) | G2145-1L | 1 L |
Product Description
1× Tris-Glycine Transfer buffer is suitable for wet or semi-dry transfer of Western-Blot experiments.
The main components of this product are 20 mM Tris, 192 mM glycine, pH 8.2-8.6 at 25.
Storage and Shipping Conditions
Ship and store at room temperature, valid for 12 months.
Assay Protocol
1. Ready to use, no need to dilute.
2. For conventional small to medium molecular weight proteins (25-70 kDa), the recommended conditions for transfer are 300 mA for about 30 min.
3. For conventional large molecular weight proteins, the transfer time can be extended as appropriate, with a recommended transfer time of 300 mA for 30-60 min.
4. For proteins with particularly large molecular weight, it is recommended to extend the transfer time to 300 mA for 60-90 min or longer, and an appropriate amount of SDS can be added to the transmembrane solution to the final concentration of about 0.025-0.1% to enhance the release of proteins from the gel. At the same time, due to the long transfer time, attention should be paid to the effect of the cooling module, a new cooling module can be replaced halfway if necessary.
Note
1. Please use it as soon as possible after opening the lid.
2. The normal color of this product is colorless and transparent, if it appears light brown or yellowish brown, it should be discarded promptly.
3. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
