Ice-Bath Free Fast Film Transfer Buffer (Powder), 1 L

Product Information

Product Description

This product is a concentrated ice-bath free fast transfer buffer, a safe and harmless buffer for wet rapid membrane transfer in Western Blot, which can efficiently and rapidly transfer proteins to the transfer membrane; without the use of methanol, it can complete the membrane transfer process within 15-30 min.

Safe and non-toxic: This product does not contain toxic and harmful components, and there is no need to use highly toxic methanol and other reagents.

This transfer buffer has the same or better results than conventional transfer buffer. 

Rapid transfer without ice bath: With rapid transfer buffer, the transfer of proteins from gels to transfer membranes such as PVDF or nitrocellulose (NC) membranes can be carried out at a constant flow membrane of 400 mA for 15-30 min, with lower heat production than with conventional transfer buffer.

Storage and Shipping Conditions

Ship and store at room temperature; Valid for 12 months.

Assay Protocol

Take 100 mL of concentrated ice-bath free fast transfer buffer, then add 600 mL of deionized or distilled water and mix well, then continue to add 200 mL of anhydrous ethanol and mix well, and finally replenish the deionized or distilled water to be fixed to 1 L, and then mix well to obtain the 1× working solution. 

Complete the sandwich structure required for transfer, from the negative electrode (black side) to the positive electrode (red side) in the order of sponge, filter paper, gel, transfer membrane, filter paper, sponge, and then transfer to the tank, add ice-bath free fast transfer buffer, set a constant current of 400 mA, transfer time of 15-30 min (this condition is for 1.0mm thick gel, if the gel thickness is 0.75mm , appropriately reduce the transfer time; If the gel thickness is 1.5 mm, extend the transfer time appropriately) to complete the protein transfer.

Note

1. Please use the working solution as soon as possible after preparation. It is recommended that the reagents be diluted with pure water for laboratory use (G4701-500ML).

2. This product is suitable for the transfer of PAGE gel of Tris-Gly, Bis-Tris and other buffer systems.

3. PVDF membrane should be infiltrated with absolute ethanol for about 30 s before use.

4. It does not require ice bath for transfer within 30 min. If the transfer is over 30 min, the ice bath is required.

5. Due to the different brand and model of the power supply used for the transfer, the maximum current and the power varies greatly, some power supplies set at a constant current of 400 mA will exceed the maximum power and overload protection will occur, so it is necessary to adjust the current downwards or use a power supply with more power.

6. The temperature of the transfer buffer, gel parameters (such as thickness, acrylamide concentration, ion concentration), filter paper thickness, volume of transfer buffer and times of use of transfer buffer may affect the current and voltage; In case of overload, please reduce the current and extend the transfer time.

7. The normal color of this product is colorless and transparent. If light brown or yellowish brown appears, it should be discarded promptly.

8. This product may have crystal precipitation in the case of low ambient temperature, this is a normal phenomenon, you can moderate heating, magnetic stirring to help dissolve, to be completely dissolved before dilution and use.

For Research Use Only!

https://www.servicebio.com/goodsdetail?id=29845