2x SDS-PAGE protein loading buffer (odorless, reduced type), 1 ml

Product Information

Product Description/Introduction

This product, 2×SDS-PAGE Protein Loading Buffer (Odorless, Reducing), uses the reducing properties and dithiothreitol (DTT) or mercaptoethanol (odor) similar substances, and no smell, water solubility is more stable, the use of the effect and the conventional SDS-PAGE protein sampling buffer has no significant difference, so it makes the operation of protein sampling safer and healthier.

Storage and Shipping Conditions

Ship with wet ice; Store at -20°C; Valid for 12 months.

Product Components

Assay Protocol/Procedures

1. Dissolve 2×SDS-PAGE Protein Loading Buffer in a water bath at room temperature or 37°C before use, store at room temperature immediately after dissolution in a water bath, avoid prolonged placement in a water bath, and store at -20°C after use.

2. Add the appropriate volume according to the ratio of protein sample: 2× SDS-PAGE Protein Sampling Buffer = 1:1. Note: This product can also be used directly for lysis of cell or tissue samples after dilution to 1×. 

3. Heat at 95°C in a water or metal bath for 10 min to fully denature the proteins.

4. The samples can be loaded directly after reaching to room temperature;

5. Electrophoresis can be stopped until the blue dye reaches the bottom of the gel.

Notes

1. Dissolve completely before use, and store at -20 immediately after use.

2. Avoid direct contact between reagents and skin.

3. For your safety or health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!

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