5x Protein Loading buffer (Non-Reducing), 1 ml
Attribute:
SKU : G2030-1ML
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Description/Introduction
This product is a 5x concentrated protein loading buffer with Bromophenol Blue as the dye for the electrophoretic loading of non-reduced SDS-PAGE protein samples. It contains Tris-HCl buffer, glycerol, SDS and bromophenol blue.
Storage and Shipping Conditions
Ship with wet ice, store at -20, valid for 12 months.
Product Components
Component | G2030-1ML |
5× SDS-PAGE Loading Buffer (Non-Reduced) | 1 mL |
Manual | 1 pc |
Assay Protocol/Procedures
1. Mix well 1 μL of 5× SDS-PAGE Loading Buffer (non-reducing type) per 4 μL of protein sample .
2. Heat in a metal or boiling water bath for 3-5 minutes to fully denature the protein sample.
3. Cool to room temperature and load on the gels.
Note
1. Ensure that the product is fully re-warmed before use.
2. For your safty and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
