SKU : G2032-1ML
Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
2 x SDS-PAGE Loading Buffer (Non-Reduced) | G2032-1ML | 1 mL |
Product Description/Introduction
The 2× SDS-PAGE Loading Buffer (Non-Reduced) containing Tris-HCl buffer (63 mM, pH6.8), glycerol (10%), SDS (2%) and bromophenol blue (0.0025%), is used for electrophoretic loading of non-reducing SDS-PAGE protein samples.
Storage and Shipping Conditions
Ship with wet ice, store at -20, valid for 12 months.
Product Components
Component | G2032-1ML |
2× SDS-PAGE Loading Buffer (Non-Reduced) | 1 mL |
manual | 1 pc |
Assay Protocol/Procedures
1. Mix well 1 μL of 1× SDS-PAGE Loading Buffer (non-reduced) per 1 μL of protein sample .
2. Heat to 95 for 3-5 minutes to fully denature the protein sample.
3. Cool to room temperature and load on the gels.
Note
1. The product should be returned to room temperature before use.
2. For your safety or health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
