Animal Testicular Tissue Fixative Solution

Product Information

Description

In histopathology, the tissue must be promptly and effectively fixed before subsequent embedding and sectioning can be completed. Organization fixed role is to maintain the inherent morphology and structure of organization, cell, prevent bacteria corrosion and tissue autolysis, save the inherent material inside the cell, the cell protein coagulation, as far as possible to reduce or end of endogenous and exogenous enzyme reaction, keep the cell or tissue basically like living material, also can make the organization hardening, easy to repair the cut of the organization. For certain infectious specimens, can prevent the spread of disease; Preserve the gross specimen. Finally, fixation can enhance the effect of staining. Formaldehyde is the most commonly used fixative, with strong penetration, uniform fixation, tissue shrinkage and appropriate hardness, which is suitable for the fixation of most tissue samples.

The traditional fixation method of testicular tissue is Bouin's fixation solution, but this fixation solution is acidic and has a great influence on antigen and nuclear staining, which is significantly affected. The testicular tissue fixed with paraformaldehyde solution was prone to interstitial loss. This fixative is specially used for the fixation of animal testis tissue with fast penetration, which can keep the structure of the tissue intact, retain most of the antigen, and do not affect the staining of the nucleus, and do not affect the detection of IHC or IF. The main components of this product are formaldehyde solution, ethanol and glacial acetic acid.

Storage and Handling Conditions

Transport at room temperature; Store in a cool and dry place, away from direct sunlight, valid for 24 months.

Assay Protocol

Mice testicular tissue was immediately put into sufficient amount of fixative solution, fixed at room temperature for 20-24 h, and then transferred into 70% ethanol for preservation.

The testicular tissue of rats and other animals is relatively large. In order to ensure thorough penetration, the tissue can be cut into appropriate size with a sharp blade, or the outer membrane can be punctured gently with a needle and then put into a sufficient amount of fixing solution, fixed for 20-24 h, not more than 48 h as far as possible, and then transferred into 70% ethanol for preservation.

Note:

1. Use the product in a well-ventilated environment with proper protection to avoid inhalation.

2. The tissue fixation time should be controlled within 48 h, and then transferred to 70% alcohol for storage or transportation, otherwise the tissue structure or protein immunodetection results may be affected.

3. Please tighten the cap in time after use to prevent volatile active ingredients.

4. For your safety and health, please wear a lab coat and disposable gloves when operating.

Images：

A：Mouse testis, fixed for 20h, paraffin sections were stained with HE，40X。

B：Mouse testis, fixed for 20h, paraffin sections were stained with HE，400X。

　C：Mouse testis, fixed for 20h, paraffin section was used to detect GluT3 by IHC (GB11294)，400X。

For Research Use Only!

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