Single Cell Sequencing
Introduction
Dissecting genomic, transcriptomic and epigenomic cellular heterogeneity using single cell Sequencing is essential for understanding biological system. With 10x chromium Gemcode technology system, Macrogen provides a high resolution of individual cell copy number variation, gene expression, cell surface protein expression, chromatin accessibility and immune diversity (TCR, BCR).
Dissecting genomic, transcriptomic and epigenomic cellular heterogeneity using single cell Sequencing is essential for understanding biological system. With 10x chromium Gemcode technology system, Macrogen provides a high resolution of individual cell copy number variation, gene expression, cell surface protein expression, chromatin accessibility and immune diversity (TCR, BCR).
Data Analysis*
Single Cell RNA Sequencing
- Standard Data Analysis
- Gene expression and cell clustering analysis
- Simultaneous single cell analysis of gene expression, immune profiling and cell surface protein expression
- Advanced Data Analysis
- Sample aggregation
- Gene expression and cell clustering analysis (Seurat_2.3.4)
- Cell identification
- Differentially Expressed Genes (DEGs) analysis
- Gene Ontology analysis
- Trajectory analysis
Single Cell immune Profiling
- Standard Data Analysis
- Paired full length V(D)J gene sequence identification
- Clonality and diversity of TCR and/or BCR
Single Cell Surface protein Profiling
- Standard Data Analysis
- Surface protein expression and cell clustering analysis
Single Cell AT AC Sequencing
- Standard Data Analysis
- Chromatin accessibility profiles and cell clustering analysis
Single Cell CNV Profiling
- Standard Data Analysis
- Chromatin accessibility profiles and cell clustering analysis
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