Recombinant Human IL-7 Protein (His-tag)

Gene Name Synonym

IL-7, IL7, Interleukin-7

Species

Human

Accession

P13232

Source

HEK293 Cells

SPECIFICATIONS

Amino acid sequence

Met1-His117 is expressed with a 10×His tag at the C terminus

Molecular Weight

21.6 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin-7 (IL-7) was discovered in 1980s. It is one of the members of IL-2 superfamily. IL-7 is a cytokine that has been known since long in immunology, mainly regarding its effects on T-cells and B-cells. IL-7 has been demonstrated to be necessary for both B-cell and T-cell proliferation and lack of IL-7 causes immature immune cell arrest. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL-7 plays in aging may permit parsimonious preventative or therapeutic solutions for diverse conditions.

Data

SDS-PAGE

Bioactivity

Testing in progress

Recombinant Human IL-7 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-30 kDa.