Recombinant Mouse BDNF Protein (His-tag)

Gene Name Synonym

Brain-derived Neurotrophic Factor

Species

Mouse

Accession

P21237

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ala19-Arg249 is expressed with a 10×His tag at the C terminus

Molecular Weight

27.5 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Brain-derived neurotrophic factor (BDNF) is a well-studied growth factor that serves many critical functions within the central nervous system (CNS). BDNF has a role in processes such as neuronal maturation, synapse formation and synaptic plasticity among others in the brain.BDNF is a member of the neurotrophin family that includes nerve growth factor (NGF), neurotrophin-3 (NT3), and neutorophin-4 (NT4). BDNF is widely expressed in the CNS and can exert profound effects on development, morphology, and synaptic plasticity and function in the brain.

Data

SDS-PAGE

Bioactivity

Recombinant Mouse BDNF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using C6 cells. The ED50 for this effect is 0.1-1 µg/mL.